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Journal: Life Science Alliance
Article Title: tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming
doi: 10.26508/lsa.202302285
Figure Lengend Snippet: (A) The workflow of tRF and tiRNA sequencing and analysis. (B) The principal component analysis of tRF and tiRNA expression in four pairs of PTC tissues (T) and adjacent normal tissues (AT). (C) The correlation coefficient analysis of tRF and tiRNA expression in tumor tissues and ATs. (D) Venn diagram of tRFs and tiRNAs detected only in tumor tissues (red region), only in ATs (blue region), and in both tissues (purple region). (E) Distributions of different tRFs and tiRNAs in tumor tissues and ATs. (F) Heatmap of differentially expressed tRFs and tiRNAs in the two groups. (G) Volcano plot of down-regulated tRFs and tiRNAs (green dots) and up-regulated tRFs and tiRNAs (red dots) in tumor tissues compared with ATs. Fold change ≥ 1.5 and P -value < 0.05. (H) Differentially expressed tRFs and tiRNAs were separately calculated in PTC, and tRF-5c was the main down-regulated type (13/20). (I) Relative expression of tRF-30 in 30 pairs of tumor tissues and ATs was detected by qRT–PCR. (J) Relative expression of tRF-30 was detected by qRT–PCR in normal thyroid epithelial cells (Nthy-ori 3-1) and four PTC cell lines (BCPAP, TPC1, KTC1, and NPA87). Data were shown as mean ± SD. ( t test and Mann–Whitney U test, * P < 0.05, ** P < 0.01, and *** P < 0.001). Quantitative results were based on three independent experiments. Source data are available for this figure.
Article Snippet: The human PTC cell lines (BCPAP, TPC1, KTC1, NPA87) and the normal
Techniques: Sequencing, Expressing, Quantitative RT-PCR, MANN-WHITNEY